5XS0
Structure of a ssDNA bound to the outer DNA binding site of RAD52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.224, 164.408, 166.087 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 48.529 - 3.000 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kn0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.571 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.630 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 107806 | 5281 |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.997 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | lithium citrate, PEG 3,350 |
