5XRR

Crystal structure of FUS (54-59) SYSSYG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-28
Detector	RAYONIX MX-225
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	28.240, 30.270, 4.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.649 - 1.503
R-factor	0.2098
Rwork	0.204
R-free	0.24070
RMSD bond length	0.007
RMSD bond angle	1.130
Data scaling software	XSCALE
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.649	20.649	1.600
High resolution limit [Å]	1.500	10.000	1.500
Rmerge	0.422	0.248	0.821
Rmeas	0.453	0.270	0.881
Total number of observations	9186
Number of reflections	1197	6	194
<I/σ(I)>	3.37	6.17	1.52
Completeness [%]	94.3	100	75.8
Redundancy	7.674	6.667	7.345
CC(1/2)	0.972	0.998	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	2.5 M NaCl, 0.1 M imidazole pH 8.0, 0.2 M zinc acetate