5XR9

Crystal structure of Human Hsp90 with FS6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 2 2 2
Unit cell lengths	67.225, 90.524, 98.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.612 - 1.501
R-factor	0.1842
Rwork	0.184
R-free	0.19530
RMSD bond length	0.006
RMSD bond angle	1.057
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.612
High resolution limit [Å]	1.501
Number of reflections	48036
<I/σ(I)>	10.7
Completeness [%]	99.9
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20-25%(w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate, pH 6.5