5XR6
Crystal structure of RabA1a in complex with GppNHp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 37.634, 48.416, 68.508 |
Unit cell angles | 88.53, 74.15, 84.37 |
Refinement procedure
Resolution | 27.183 - 2.600 |
R-factor | 0.2092 |
Rwork | 0.203 |
R-free | 0.26530 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.127 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 14606 |
<I/σ(I)> | 28.6 |
Completeness [%] | 96.5 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M MES (pH5.5), 10 mM magnesium acetate, 2.2 M ammonium sulfate |