5XQW
Catalytic antibody 7B9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-25 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.942, 61.904, 70.959 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.200 |
| R-factor | 0.19453 |
| Rwork | 0.187 |
| R-free | 0.26082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kb5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 19632 | |
| <I/σ(I)> | 12.6 | 2.4 |
| Completeness [%] | 98.8 | 95.5 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100mM Tris HCl, 15%(w/v) PEG 1000, 5% 2-methyl-2,4-pentanediol |
