5XQV
Crystal structure of Notched-fin eelpout type III antifreeze protein A20L mutant (NFE6, AFP), P21 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.475, 33.735, 34.391 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 31.880 - 0.970 |
| R-factor | 0.13 |
| Rwork | 0.129 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xqn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.068 | 0.317 |
| Number of reflections | 34361 | |
| <I/σ(I)> | 28.4 | 3.1 |
| Completeness [%] | 95.5 | 86 |
| Redundancy | 2.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.3 M Ammonium sulfate, 0.1 M Na-Citrate pH5.0 |
