5XQ1

Structural basis of kindlin-mediated integrin recognition and activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	75.973, 75.973, 387.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.986 - 2.954
R-factor	0.2216
Rwork	0.220
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.076	0.038	0.987
Rmeas	0.083	0.042	1.068
Rpim	0.032	0.017	0.402
Total number of observations	186136
Number of reflections	28446
<I/σ(I)>	5.6
Completeness [%]	99.8	97.7	99.9
Redundancy	6.5	5.6	6.8
CC(1/2)		0.998	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v pentaerythritol propoxylate