5XPX
Structure elucidation of truncated AMS3 lipase from an Antarctic Pseudomonas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.753, 94.693, 126.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.770 |
| R-factor | 0.22463 |
| Rwork | 0.222 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fdm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.689 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.820 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Number of reflections | 27263 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M HEPES sodium pH 7.5, 0.8 M Potassium sodium tartrate tetrahydrate |
