5XPA
Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 9'U10' on the target strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97892 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 109.134, 109.134, 184.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.150 - 2.900 |
R-factor | 0.218 |
Rwork | 0.215 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpy |
RMSD bond length | 0.006 |
RMSD bond angle | 0.979 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 25323 |
<I/σ(I)> | 46.5 |
Completeness [%] | 99.8 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 308 | 3.1 M NH4-acetate, 0. 1 M Bis-Tris propane, pH 7.3 |