5XP8

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 4A5 on the guide strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	2014-12-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 41 3 2
Unit cell lengths	202.140, 202.140, 202.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.200 - 3.100
R-factor	0.217
Rwork	0.214
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpy
RMSD bond length	0.022
RMSD bond angle	1.633
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	25862
<I/σ(I)>	9.7
Completeness [%]	98.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		308	2.6 M Na-acetate, pH 7.0, 0.1M Tris-Cl, pH 7.4 and 0.2 M glycine