5XP0
Crystal structure of master biofilm regulator CsgD regulatory domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.758, 57.758, 207.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.355 - 2.000 |
R-factor | 0.209 |
Rwork | 0.206 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.286 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.355 |
High resolution limit [Å] | 2.000 |
Number of reflections | 24586 |
<I/σ(I)> | 15.7 |
Completeness [%] | 99.1 |
Redundancy | 24.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | (1) 1.6M magnesium sulfate heptahydrate, 0.1M MES, pH 6.5 (2) 1.5M ammonium sulfate, 0.1M BIS-TRIS propane pH 7.0 |