5XOG

RNA Polymerase II elongation complex bound with Spt5 KOW5 and Elf1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	155.250, 159.910, 268.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.552 - 3.000
R-factor	0.1956
Rwork	0.195
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.552	49.552	3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.110	0.026	1.289
Rmeas	0.119	0.028	1.405
Number of reflections	134084	1624	9841
<I/σ(I)>	15.48	50.89	1.52
Completeness [%]	99.9	97.2	99.9
Redundancy	6.733	5.982	6.4
CC(1/2)	0.998	0.999	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	50 mM potassium malonate (pH 6.0), 150 mM Tris malonate (malonic acid titrated with Tris(hydroxymethyl) aminomethane, pH 6.0), 6.67% (v/v) glycerol, 6.67% (w/v) trehalose dihydrate and 10% PEG20000