5XNS

Crystal structure of the Smc head domain with an extended coiled coil bound to the C-terminal domain of ScpA derived from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	93.304, 150.003, 51.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.932 - 2.010
R-factor	0.2082
Rwork	0.207
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i99
RMSD bond length	0.008
RMSD bond angle	0.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.010
Number of reflections	45242
<I/σ(I)>	31.6
Completeness [%]	92.8
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1M Ammonium citrate dibasic, 0.1M Sodium acetate trihydrate pH 4.6, 0.3M NDSB-195