5XNQ
Crystal structures of human SALM5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 152.601, 152.601, 93.822 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.875 - 2.802 |
R-factor | 0.2489 |
Rwork | 0.248 |
R-free | 0.26890 |
Structure solution method | SIRAS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.805 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.056 | 0.588 |
Number of reflections | 16115 | 786 |
<I/σ(I)> | 36.875 | 3.4 |
Completeness [%] | 98.8 | 99.2 |
Redundancy | 6.4 | 6.6 |
CC(1/2) | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2% PEG 3000, 0.1 M sodium acetate, 0.2 M lithium sulfate |