5XN9

Crystal structure of a secretary abundant heat soluble (SAHS) protein from Ramazzottius varieornatus (from monomer sample)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-16
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	53.866, 57.505, 111.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.259 - 1.449
R-factor	0.1457
Rwork	0.145
R-free	0.17190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.449	1.449
Rmeas	0.077	0.933
Number of reflections	62308	3067
<I/σ(I)>	42.3	1.5
Completeness [%]	99.9	100
Redundancy	11.8	7.3
CC(1/2)		0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	12 mg/mL protein, 100 mM HEPES pH 7.8, 150 mM MgCl2, 1 mM ZnSO4, 3.0% v/v 2-propanol, and 21% v/v PEG 600