5XN1
HIV-1 reverse transcriptase Q151M:DNA:entecavir-triphosphate ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 284.707, 284.707, 95.793 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.018 - 2.446 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d3g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.901 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.446 | 2.450 |
| Rpim | 0.113 | |
| Number of reflections | 106838 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20 mM bis-Tris-HCl pH 6.0, 60 mM di-ammonium hydrogen citrate, 20 mM MgCl2, 1.5-3% PEG 6000, 2-4% sucrose, 4-8% glycerol |
