5XMH

Crystal structure of an IgM rheumatoid factor YES8c in complex with IgG1 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	189.895, 189.895, 79.602
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 2.800
R-factor	0.223
Rwork	0.220
R-free	0.27200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.074	0.355
Number of reflections	77576	3825
<I/σ(I)>	19.1
Completeness [%]	98.7	95.1
Redundancy	4.6	3.8
CC(1/2)		0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Tris-HCl, sodium acetate, PEG 4000