5XMF
Crystal structure of feline MHC class I for 2,1 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.421, 83.379, 121.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.21533 |
| Rwork | 0.213 |
| R-free | 0.25521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pwv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.157 |
| High resolution limit [Å] | 2.100 | 2.103 |
| Rmerge | 0.542 | 0.542 |
| Number of reflections | 27016 | |
| <I/σ(I)> | 7.31 | 30.971 |
| Completeness [%] | 99.5 | 99.55 |
| Redundancy | 12.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG/Ion solution No. 23 (0.2M ammonium formate and 20%(w/v) polyethylene glycol 3,350) |
