5XLY

Crystal structure of CheR1 in complex with c-di-GMP-bound MapZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	I 1 2 1
Unit cell lengths	79.440, 57.410, 104.639
Unit cell angles	90.00, 100.16, 90.00

Refinement procedure

Resolution	19.450 - 1.763
R-factor	0.2091
Rwork	0.208
R-free	0.22850
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_2719)

Data quality characteristics

	Overall
Low resolution limit [Å]	68.360
High resolution limit [Å]	1.760
Number of reflections	44834
<I/σ(I)>	4.39
Completeness [%]	97.6
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	15% 2-propanol 0.1M Sodium citrate 5.0 10% PEG 10000