5XKU
Crystal structure of hemagglutinin globular head from an H7N9 influenza virus in complex with a neutralizing antibody HNIgGA6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.983, 70.983, 283.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.318 - 1.780 |
| R-factor | 0.2075 |
| Rwork | 0.206 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n5j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.931 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.318 | 1.890 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.058 | |
| Number of reflections | 70029 | 11241 |
| <I/σ(I)> | 17.22 | |
| Completeness [%] | 98.8 | 97.4 |
| Redundancy | 7.07 | 7.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Calcium acetate hydrate, 20% w/v Polyethylene glycol 3,350 |
