5XJZ
Structure of DNA1-Ag complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.236, 42.546, 25.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.230 - 0.980 |
| R-factor | 0.12399 |
| Rwork | 0.124 |
| R-free | 0.13065 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.276 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.010 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.492 | |
| Number of reflections | 24202 | 1694 |
| <I/σ(I)> | 31 | 2.2 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 6.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v MPD, 40mM Sodium cacodylate pH 7.0, 12mM Spermine tetra-HCl, 80mM NaCl |
