5XJX
Pre-formed plant receptor ERL1-TMM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-07 |
| Detector | MAC Science DIP-320 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 116.172, 114.395, 191.952 |
| Unit cell angles | 89.57, 90.46, 59.86 |
Refinement procedure
| Resolution | 43.249 - 3.055 |
| R-factor | 0.2547 |
| Rwork | 0.254 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mn8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.702 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.090 |
| High resolution limit [Å] | 3.050 | 3.055 |
| Number of reflections | 158048 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 4% v/v Tacsimate pH 6.0, 10% w/v Polyethylene glycol 3350 |
