5XJM

Complex structure of angiotensin II type 2 receptor with Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-25
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	465.390, 48.650, 55.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.386 - 3.200
R-factor	0.2304
Rwork	0.226
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yay 1m6t
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.386	3.380
High resolution limit [Å]	3.200	3.200
Number of reflections	22033	3410
<I/σ(I)>		1.61
Completeness [%]		99.9
CC(1/2)		0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	34%(v/v) PEG 300, 0.1 M HEPES pH 7.0, 0.5%(v/v) Tacsimate (pH7.0)