5XJ9
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the orthophosphate form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.339, 97.986, 83.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.730 - 1.830 |
| R-factor | 0.2099 |
| Rwork | 0.205 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.730 | 1.890 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.175 | 0.889 |
| Rpim | 0.075 | 0.433 |
| Number of reflections | 21613 | |
| <I/σ(I)> | 7.02 | 2.28 |
| Completeness [%] | 95.3 | 86.02 |
| Redundancy | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 277 | 1 mol% palmitoyl lysophosphatidic acid, 0.4 M Na2HPO4, 24% PEG 400, 0.1M HEPES pH 7.0 |
