5XJ7
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the acyl phosphate form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.224, 65.599, 84.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.573 - 1.772 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.220 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.064 | 0.798 |
| Rpim | 0.029 | 0.429 |
| Number of reflections | 25689 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 98.4 | 97.1 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 277 | 7.8 monoacylglycerol (7.8 MAG), 0.6M potassium fluoride, 22-26% PEG 400, 0.1M Tris HCl pH 8.0 |
