5XJ5
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the monoacylglycerol form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.675, 83.437, 107.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.582 - 1.481 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.21280 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.054 | 0.893 |
| Rpim | 0.025 | 0.428 |
| Number of reflections | 43520 | |
| <I/σ(I)> | 16.6 | 1.7 |
| Completeness [%] | 98.4 | 94.4 |
| Redundancy | 5.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 3.8 | 293 | 7.8 monoacylglycerol (7.8 MAG), 0.1 M ammonium sulphate, 25-30% triethylene glycole, 0.1 M glycine/HCl pH 3.8 |
