5XJ2
Structure of spRlmCD with U747 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.384, 94.916, 164.164 |
| Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.840 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.720 | 2.850 |
| Rmerge | 0.366 | 0.089 | 0.991 |
| Rmeas | 0.409 | 0.099 | 1.126 |
| Rpim | 0.180 | 0.043 | 0.525 |
| Total number of observations | 215130 | ||
| Number of reflections | 45742 | ||
| <I/σ(I)> | 2.9 | ||
| Completeness [%] | 99.9 | 100 | 99.9 |
| Redundancy | 4.7 | 4.7 | 4.1 |
| CC(1/2) | 0.992 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 50mM MES 0.1M ammonium sulfate 0.01 M magnesium chloride 20%PEG8000 |
