5XIS
Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-04-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.273, 66.291, 74.173 |
| Unit cell angles | 76.47, 79.29, 80.49 |
Refinement procedure
| Resolution | 43.286 - 1.780 |
| R-factor | 0.2198 |
| Rwork | 0.218 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fid |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 56388 | |
| <I/σ(I)> | 32.7 | 1.4 |
| Completeness [%] | 92.9 | 92 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 15% PEG3350, 0.1 M Tris-HCl (pH 8.5), 100 mM MgCl2 |
