5XHP

Transferase with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	50.116, 50.116, 201.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.402 - 2.800
R-factor	0.28994
Rwork	0.288
R-free	0.31864
RMSD bond length	0.011
RMSD bond angle	1.527
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	13266
<I/σ(I)>	2.6
Completeness [%]	99.8
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	290	0.2M ammonium acetate, 0.1M Bis-Tris[pH5.5], 25% PEG2250