5XHN
Crystal structure of Frog M-ferritin K104E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-01-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.210, 184.210, 184.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.270 - 1.630 |
| R-factor | 0.16446 |
| Rwork | 0.163 |
| R-free | 0.19158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.283 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.270 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 33970 | |
| <I/σ(I)> | 33.8 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2.0 M MgCl2 and 100 mM Bicine (pH 9.0) |
