5XH8
Aspergillus kawachii beta-fructofuranosidase complexed with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.799, 108.799, 152.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.110 - 2.100 |
| R-factor | 0.17253 |
| Rwork | 0.171 |
| R-free | 0.20245 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.426 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.480 |
| Number of reflections | 61305 | |
| <I/σ(I)> | 53.6 | 7.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.1 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, 2.6 M sodium formate |
