5XGX

Crystal structure of colwellia psychrerythraea strain 34H isoaspartyl dipeptidase E80Q mutant complexed with beta-isoaspartyl lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 4 21 2
Unit cell lengths	107.878, 107.878, 155.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.330
R-factor	0.19177
Rwork	0.189
R-free	0.24636
RMSD bond length	0.016
RMSD bond angle	1.840
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	2.330
Number of reflections	36407
<I/σ(I)>	31.8
Completeness [%]	97.1
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris propane pH 7.0 1 M ammonium citrate tribasic pH 7.0