5XFQ
Ternary complex of PHF1, a DNA duplex and a histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-01-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.243, 62.550, 97.338 |
| Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
| Resolution | 47.414 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xfn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.523 |
| Number of reflections | 32456 | |
| <I/σ(I)> | 17.9 | 1.8 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05M Bis-Tris at pH 6.5, 0.05M Ammonium Sulfate, 30% pentaerythritol ethoxylate (15/4 EO/OH) |
