5XFO
Structure of the N-terminal domains of PHF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.467, 66.772, 76.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.338 - 1.900 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.22710 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.132 | 0.807 |
| Number of reflections | 25789 | |
| <I/σ(I)> | 18.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Tris-pH 8.0, 10% PEG 3350, 22% ethylene glycerol |
