5XF6
Nucleosome core particle with an adduct of a binuclear RAPTA (Ru-arene-phosphaadamantane) compound having an ethylenediamine linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.840, 109.729, 182.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 2.630 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.454 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 2.770 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 60682 | |
| <I/σ(I)> | 31.8 | |
| Completeness [%] | 94.5 | 71.6 |
| Redundancy | 5.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate , pH 6.0 |
