5XEZ
Structure of the Full-length glucagon class B G protein-coupled receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-08-20 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.610, 245.330, 96.150 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 31.780 - 3.000 |
| R-factor | 0.21123 |
| Rwork | 0.210 |
| R-free | 0.24327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l6r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.414 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 67598 |
| <I/σ(I)> | 4.8 |
| Completeness [%] | 100.0 |
| Redundancy | 198 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100mM HEPES, pH7.0, 300mM potassium phosphate monobasic, 25% PEG500DME, 100mM gly-gly-glycine |
