5XEU
crystal structure of Hcp2 from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.645, 147.786, 85.687 |
| Unit cell angles | 90.00, 123.74, 90.00 |
Refinement procedure
| Resolution | 32.799 - 3.000 |
| R-factor | 0.2391 |
| Rwork | 0.237 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y12 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.530 |
| Rpim | 0.054 | 0.395 |
| Number of reflections | 21754 | 1098 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 2.2M Ammonium sulfate, 0.1M ammonium acetate, pH 5.6 |
