5XEI
Crystal structure of the Smc head domain with a coiled coil and joint derived from Pyrococcus yayanosii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.320, 50.836, 122.057 |
| Unit cell angles | 90.00, 118.09, 90.00 |
Refinement procedure
| Resolution | 39.625 - 2.599 |
| R-factor | 0.2145 |
| Rwork | 0.207 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xns |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.599 |
| Number of reflections | 18871 |
| <I/σ(I)> | 13.7 |
| Completeness [%] | 92.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 7% PEG 3350, 4% Tacsimate pH4.0 |
