5XE3
Endoribonuclease in complex with its cognate antitoxin from Mycobacterial species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.473, 70.018, 76.754 |
| Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3se5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.677 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 23785 |
| <I/σ(I)> | 30.21 |
| Completeness [%] | 95.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 293 | 0.2 M calcium acetate, 0.1 M Tris-HCl pH 7.0, 20% (w/v) polyethylene glycol 3000 |
