5XDT
Staphylococcus aureus FtsZ 12-316 complexed with TXA707
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.553, 51.193, 86.449 |
| Unit cell angles | 90.00, 108.68, 90.00 |
Refinement procedure
| Resolution | 40.950 - 1.300 |
| R-factor | 0.1377 |
| Rwork | 0.136 |
| R-free | 0.16810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 3VOA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.746 |
| Data reduction software | HKL |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.049 | 0.035 | 0.760 |
| Rmeas | 0.053 | 0.039 | 0.823 |
| Rpim | 0.020 | 0.016 | 0.311 |
| Total number of observations | 503253 | ||
| Number of reflections | 71548 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 99.5 | 90.8 | 100 |
| Redundancy | 7 | 6.4 | 6.9 |
| CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 100mM Tris-HCl, 41% w/v PEP629, 300mM KCl |
