5XCJ

Crystal structure of Vps29 single mutant (D62A) from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-25
Detector	MAR CCD 165 mm
Wavelength(s)	0.95
Spacegroup name	P 1 21 1
Unit cell lengths	35.428, 67.364, 77.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.729 - 1.859
R-factor	0.2108
Rwork	0.206
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xce
RMSD bond length	0.007
RMSD bond angle	1.305
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	30853
<I/σ(I)>	8.5
Completeness [%]	100.0
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	200 mM Sodium Acetate, 100mM Sodium cacodylate pH 6.5, 30% PEG 8K