5XCH

Crystal structure of Wild type Vps29 complexed with Zn+2 from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-23
Detector	MAR CCD 165 mm
Wavelength(s)	1.2
Spacegroup name	P 32
Unit cell lengths	47.310, 47.310, 148.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.900 - 2.850
R-factor	0.2879
Rwork	0.287
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xce
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.900
High resolution limit [Å]	2.850
Number of reflections	8561
<I/σ(I)>	21
Completeness [%]	98.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	10 % w/v PEG 20000, 20 % v/v PEGMME 550, 0.02 M of alcohol MIX, 0.1 M MES/Imidazole pH 6.5