5XC3
Crystal structure of Acanthamoeba polyphaga mimivirus Rab GTPase in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.575, 66.726, 69.754 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.691 - 1.497 |
R-factor | 0.1807 |
Rwork | 0.179 |
R-free | 0.19920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tu4 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.938 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.497 |
Number of reflections | 27374 |
<I/σ(I)> | 51.9 |
Completeness [%] | 99.7 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium iodide and 20% (w/v) polyethylene glycol 3350 |