5XBQ
Peroxiredoxin from Pyrococcus horikoshii (6m mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.583, 98.598, 137.571 |
| Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
| Resolution | 39.730 - 2.250 |
| R-factor | 0.19533 |
| Rwork | 0.193 |
| R-free | 0.23191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w6g |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 150002 |
| <I/σ(I)> | 6.4 |
| Completeness [%] | 98.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate (pH 5.0), 0.2 M magnesium chloride, and 19% PEG400 |
