5XBM
Structure of SCARB2-JL2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-12-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.863, 75.646, 164.305 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 49.554 - 3.501 |
| R-factor | 0.2409 |
| Rwork | 0.238 |
| R-free | 0.29310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tw2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 30522 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.2 M Sodium citrate tribasic dehydrate pH 8.3, 20% w/v Polyethylene glycol 3350 |
