5XBJ

The structure of the flagellar hook junction protein HAP1 (FlgK) from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	49.501, 123.552, 92.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.971 - 2.448
R-factor	0.1834
Rwork	0.178
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d4y
RMSD bond length	0.003
RMSD bond angle	0.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.620
High resolution limit [Å]	2.450
Number of reflections	21573
<I/σ(I)>	12.8
Completeness [%]	99.8
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	30% PEG MME 2000, 0.4M AMMONIUM, ACETATE, 2% MPD, 4% 2-PROPANOL, 5% ETHYLENE GLYCOL, 0.1M SODIUM CITRATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K