5XBF
Crystal Structure of Myo7b C-terminal MyTH4-FERM in complex with USH1C PDZ3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.634, 42.537, 118.390 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 35.293 - 1.802 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.21070 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.997 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.059 | 0.031 | 0.492 |
| Rmeas | 0.071 | 0.036 | 0.595 |
| Rpim | 0.039 | 0.020 | 0.330 |
| Number of reflections | 63640 | ||
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.1 | 96.6 | 99.8 |
| Redundancy | 3 | 3 | 3 |
| CC(1/2) | 0.997 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 299 | 8% tacsimate, pH 7.0 and 20% v/w PEG 3350 |
