5XB7

GH42 alpha-L-arabinopyranosidase from Bifidobacterium animalis subsp. lactis Bl-04

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	177.954, 177.954, 375.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.368 - 2.000
R-factor	0.15704
Rwork	0.155
R-free	0.19127
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kwg
RMSD bond length	0.022
RMSD bond angle	1.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	400874	19842
<I/σ(I)>	27.7	3.8
Completeness [%]	100.0	100
Redundancy	14.9	14.6
CC(1/2)		0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	12% glycerol, 1.6 M ammonium sulfate, 0.1 M MES buffer pH 6.5