5XAY
Crystal structure of full length tylp, a tetr regulator from streptomyces fradiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.329, 71.885, 160.066 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.580 |
| R-factor | 0.22813 |
| Rwork | 0.227 |
| R-free | 0.28386 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.010 |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.620 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.542 | |
| Number of reflections | 69940 | |
| <I/σ(I)> | 2.9 | 2.9 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Ammonium citrate tribasic (pH 7.0), 20% w/v PEG 3350 |
