5XA7
Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E1-2CA2+ crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.80 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.200, 64.545, 146.220 |
| Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
| Resolution | 82.270 - 3.200 |
| R-factor | 0.2263 |
| Rwork | 0.225 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WPE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.550 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 144.750 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 25724 |
| <I/σ(I)> | 81.76 |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | PEG 2000 |
